免疫球蛋白基因组.ppt
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TheDiversityofImmunoglobulin,YanWang,Supervisor:AndrewCollinsCosupervisor:WilliamSewell,Immunoglobulin,StructuresHeavychainandlightchain(and)ConstantregionClass:IgA,IgD,IgE,IgGandIgMVariableregionAntigenbindingsite,Generationofdiversities,AID,N-Regions,Part1,Immunoglobulingermlinegenerepertoirestudy,IGHVgenerepertoire,Highlypolymorphic226reportedgenesandallelicvariants104wasreportedinerror12putativenewpolymorphisms,3havebeensequencedConclusion:TheexistingIGHVgenerepertoireisfullofincorrectlyreportedsequences.,IGKVgenerepertoireanalysis,CompilationofDatabase1863rearrangedIGKVsequencesfromtheEMBL66germlinegenesfromtheIMGTAlignmentsusingSmith-Watermanalgorithm.UnreportedIGKVallelesIGKValleleswithlownumberofunmutatedsequenceswerecollectedMultiplealignmentSharedmismatchesSequencingfromgenomicDNA,JunctionanalysisofIGKgenes,435lowmutationsequences(3IGKVmutations)wererealignedtodetermineIGKJgenesusingV-QUESTVJgeneuseageN-Region,Results,5reportedgenesmaybetheresultofsequencingerrorsIGKV1D-13*01andIGKV1-37*01maybefunctionalinsomeindividualsUnreportedpolymorphisms:IGKV1-16*02andIGKV2-30*02.TheexistingIGKVrepertoireisrelativelycompleteandaccurate,Conclusions,Notonlyistherelittlediversityinthegermlinegenesofthekappalocus,butlittlejunctionaldiversityisgeneratedduringtheprocessofgenerearrangement.Theimmunoglobulingenerepertoirecanthereforebecharacterisedashavingextraordinarydiversityintheheavychain,andlimiteddiversityinthelightchain.,Part2,Sequencingimmunoglobulingenesfromallergicandparasitisedindividuals,IgEandAllergy,NaturalfunctionofIgEParasiteinfectionsIgEandAllergicdiseaseAntigenselectioninIgETherelationshipbetweenIgEandIgG4,UnderstandingIgEmutationsinPNG,PNGIgEvsPNGIgGOtherFactorsExposuretopathogensAge,TwoQuestions,IsthereahigherlevelofmutationinantibodiesofPNGvillagers,comparedtoAustraliaurbanindividuals?Arehighernumbersofmutationsseeninolderindividuals?,Acknowledgements,AndrewCollinsWilliamSewellKatherineJacksonVivekaSinghElliePratt,BrunoGaetaJeanetteVillanuevaJamesLazenbyChristineWhittallAngelaHiggins,Thankyou!,- 配套讲稿:
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