Invitrogen中国测序通用引物序列

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1、Invitrogen中国测序通用引物序列引物名称序列(5-3)M13RCAG GAA ACA GCT ATG ACCM13FTGT AAA ACG ACG GCC AGTM13F(-47) CGC CAG GGT TTT CCC AGT CAC GACM13R(-48)AGC GGA TAA CAA TTT CAC ACA GGAM13(-96) CCC TCA TAG TTA GCG TAA CGSP6 ATT TAG GTG ACA CTA TAGT7TAA TAC GAC TCA CTA TAG GGT7 terminator TGC TAG TTA TTG CTC AGC GGT3 A

2、TT AAC CCT CAC TAA AGG GApGEX-4T-5GGG CTG GCA AGC CAC GTT TGG TGpGEX-4T-3CCG GGA GCT GCA TGT GTC AGA GGGLp1TGT ATC TTA TGG TAC TGT AAC TGGLp2CTT TAT GTT TTT GGC GTC TTC CARVp3CTA GCA AAA TAG GCT GTC CCRVp4GAC GAT AGT CAT GCC CCG CGpcDNA3.1RTAG AAG GCA CAG TCG AGGPinPoint primerCGT GAC GCG GTG CAG GG

3、C GpCMV-FTCT AAA AGC TGC GGA ATT GTpCMV-R TCCAAACTCATCAATGTATCpTRC99C-F: TTG CGC CGA CAT CAT AACpTRC99C-R: CTGCGTTCTGATTTAATCTGpCEP-F: AGA GCT CGT TTA GTG AAC CGEBV-R :GTG GTT TGT CCA AAC TCA TCpIRES2-EGFP.P5:GTA GGC GTG TAC GGT GGG AGpIRES2-EGFP.P3: AAC GCA CAC CGG CCT TAT TC3AD: AGA TGG TGC ACG AT

4、G CAC AGCMV -FCGC AAA TGG GCG GTA GGC GTGS1 CAA CGT GAA AAA ATT ATT ATT CGCS6 GTA AAT GAA TTT TCT GTA GTA GG5AOX1 GAC TGG TTC CAA TTG ACA AGC3AOX1 GCA AAT GGC ATT CTG ACA TCC-FactorTAC TAT TGC CAG CAT TGC TGCGAL4 ADTAC CAC TAC AAT GGA TGpACT2-RGTGCACGATGCACAGTTGAApB42ADF: CCA GCC TCT TGC TGA GTG GAG

5、 ATGpB42ADR: AAG CCG ACA ACC TTG ATT GGA GpEGFP-N-5: TGG GAG GTC TAT ATA AGC AGA GpEGFP-N-3: CGT CGC CGT CCA GCT CGA CCA GpEGFP-C-5: CAT GGT CCT GCT GGA GTT CGT GpEGFP-C-3 : TAT GGC TGA TTA TGA TCA GTPBV220F: AAG AAG GGC AGC ATT CAA AGPBV220R:CTG CGT TCT GAT TTA ATC TGU6ATG GAC TAT CAT ATG CTT ACC G

6、TA2.0rev primer:AGG CGA TTA AGT TGG GTA3.0rev: GAG TTA GCT CAC TCA TTA GGCS.tagGAA CGC CAG CAC ATG GAC5TriplEx2CTC CGA GAT CTG GAC GAG CRVP4GAC GAT AGT CAT GCC CCG CGRVP3 CTA GCA AAA TAG GCT GTC CCpQE30-RGTT CTG AGG TCA TTA CTG GpQE30-FTGA GCG GAT AAC AAT TTC ACpEF-FTCA AGC CTC AGA CAG TGG TTCpDSRED

7、-N1-RTGA AGC GCA TGA ACT CCT TGpDsRED-express-C1-FTCC CAC AAC GAG GAC TAC ACpCMV5R ATT ATA GAG GAC ACC TAG TCpCMV5F TTC CAA AAT GTC GTA ATA ACP5TGC GTA CTG CGG TGA TCA ACP3CTG CAA GGC GAT TAA GTT GGBAC2ACG CAC AGA ATC TAG CGC TTBAC1AAC CAT CTC GCA AAT AAA TA3BD: TAA GAG TCA CTT TAA AAT TTG TAT ACpTa

8、rget.FCCA GGA TTT TCC CAG TCA CpTarget.RGGC TTT ACA CTT TAT GCT TCT7(17base)ACA TCC ACT TTG CCT TTC TCpYes2.RTCG GTT AGA GCG GAT GTGGAL4 BDTCA TCG GAA GAG AGT AGpAS2-1.RAAG AGT TAC TCA AGA ACA AGA ApFlag-CMV-F:GGT AGG CGT GTA CGG TGGpFlag-CMV-R:GCA CTG GAG TGG CAA CTTpIRES-F:CTT ACT GAC ATC CAC TTT

9、GCpIRES.R:CAC TGC ATT CTA GTT GTG GTP1CCA GGC TTT ACA CTT TAT GCP2GCG ATT AAG TTG GGT AAC GCpLEXA-FCGT CAG CAG AGC TTC ACC ATpLEXA-RTAA AAC CTA AGA GTC ACT TTpLNCX-FAGC TCG TTT AGT GAA CCG TCA GAT CGpLNCX-RACC TAC AGG TGG GGT CTT TCA TTC CCpLXSN-FCTT GAA CCT CCT CGT TCG ACpLXSN-RGTT GCT GAC TAA TTG

10、AGA TGpSHUTTLE-CMV-FGGT CTA TAT AAG CAG AGG TGpSHUTTLE-CMV-RGTG GTA TGG CTG ATT ATG ATC AGMalE PrimerGGT CGT CAG ACT GTC GAT GAA GCCInvitrogen中国使用的测序载体和引物Primer of VectorVectorPrimer(F)Primer(R)pACTT7T3pACT2GAL4 ADpACT2-RpAS2-1GAL4 BDpAS2-1.RpB42ADpB42ADFpB42ADRpBACPAK8BAC1BAC2pBK-CMVT7T3PBS(SK/KS)/

11、M13-M13F/T7T3/M13RpBV220PBV220FPBV220RpCAMBIA 1301(1300)P1P2pCAMBIA 2300M13R(-48)M13F(-47)PCANTB5ES1/M13RS6pCAT3-enhancerRVP3此载体无反向引物pcDNA3.0CMV-F/T7SP6/BGHpcDNA3.1T7BGHpcDNA4T7/CMV-FBGHpcDNA6T7/CMV-F(J21025在T7前面）BGHpcDNAIIT7SP6pCE2.1M13FM13RpCEP4pCEP-FEBV-RpCF-TM13FM13RpCIT7（17Base）此载体无反向引物pCI-neoT

12、7（17Base）T3pCMS-EGFPT7T3pCMV-3Tag-4AT3 /PFLAG-CMV-FT7pCMV5pCMV5FpCMV5RpCMV5-FlagpCMV5FpCMV5RpCMV-MYC/HApCMV-FpCMV-RpCMV-SportM13F/T7SP6/M13RpCMV-Tag(KAN+)T3T7pCR2.1-TOPOM13F/T7M13RpCR3.1T7BGHpCS2SP6T7pDonarM13FM13RpDONR221M13FM13RpDriveT7SP6pDRIveR(KAN+)T7SP6pDsRED1-C1pDsRED-ex-C1-FpEGFP-N-3(距离很近,一般

13、不用)pDsRED2-C1(KAN+)pDsRED-ex-C1-FpEGFP-N-3pDSRED-N1(KAN+)pEGFP-N-5PDSRED-N-RpECFP-CpEGFP-C-5pECFP-C-3pECFP-N1pEGFP-N-5pEGFP-N-3pEF/myc/ERpEF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5pEGFP-C-3pEGFP-N(KAN+)pEGFP-N-5pEGFP-N-3pENTR/D-TOPO(KAN+)M13FM13RpET-*(His)(KAN+)T7T7 TerpET22(a,b,c)(AMP+)T7T7 TerpET28,30,24

14、,49(KAN+)T7T7 TerpET32(a,b,c)(AMP+)T7/S.tagT7 TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7 TerpEYFP-N1pEGFP-N-5pEGFP-N-3pEYFP-C1pEGFP-C-5pEGFP-C-3pFLAG-CMVpFLAG-CMV -FpFLAG-CMV -RpGAD424GAL4 AD3ADpGADGHGAL4 AD3ADpGADT7-RecGAL4 AD/T73ADpGAPZa-Aa-FACTOR3AOXpGBKT7(Kana+)T73BDpGEM-T(-Easy)M13F/T7SP6

15、/M13RpGEX-(*)TpGEX-4T-5pGEX-4T-3pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)PinpointTM VectorPinpoint PrimerSP6pIRESPIRES-F/T7T3/PIRES-R (J40720)pIRES2-EGFPpIRES2-EGFP.P5pIRES2-EGFP.P3pLXSNpLXSN-FpLXSN-RpMAL-c2EMalE primerM13F(-47)pMAL-C2xP5P3pMAL-p2XMalE primerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19

16、-TM13F(-47)M13R(-48)pPIC9K(AMP+、ZEO+）5AOX/a-factor3AOXpPICZa5AOX/PPICZa-F3AOXpPROEXHTAM13R(-48)无反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSilencer3.1-H1 hygroM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSP72T7SP6pSport1SP6/M13F(-47)T7/M13RpSUPERT7T3pTA2M13F/T7(优先使用M13F）T3/M13R

17、pT-AdvM13F /T7M13RpTARGET TMpTarget.F(在T7前面）/T7pTarget.RpTO-T7T7T7TERpTRC99a-cpTRC99C-FpTRC99C-RpTriPLEx25pTriPLEx2T7pTWIN-1T7T7TER(客户确认再用）pUC18(19)/118(119)M13F(-47)M13R(-48)pUCm-TM13F/T7M13RpVAX1T7BGHpWSK29T7/M13F/M13F(-47)T3/M13R/M13R(-48)pXT7T7SP6pYES2T7pYes2.RpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpRECEIVERCMV-F此载体无反向引物pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-RpSP64/65SP6此载体无反向引物pSTBlue-1T7SP6pT7Blue(R)T7M13F(-47)