试验中动物安乐死时机及准则

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1、IACUC参考资料中勤物安槊死日寺檄及?ww背景 Background中勤物安槊死日寺横及卒JW由IACUC制言丁，主要在尚未完成之86物畿生所引 起或非55期病症，而必物安槊死之状沉。These criteria have been established by the Institutional Animal Care and Use Committee, which recognizes that euthanasiais sometimes necessaryprior to the scheduled end of a study, either because of unantic

2、ipated complications, or because of the protocol itself.利0 CriteriaIACUC已睿查同意之情形(如：引起之 58期中病症且能使8&物痈苦程度减至最低)，否JW只要符合以下任一：S情沉即需将86物安槊 死。These criteria apply to all animals on experimental protocols, whether manipulated or not. Fulfillment of one criterion can constitute grounds for euthanasia. Except

3、ions are permitted only if approved by the Committee as part of the protocol review process (i.e. the clinical signs listed below are expected as part of the experiment and appropriate measures are taken to minimize pain or discomfort in the animals).1. 醴重减IS Weight loss:醴重20-25%,或是8&物出现悲病斐或消耗性症候日寺。

4、*非生畏期s&物醴重减可依a&物物房之醴重或平均年意邻重舄依at。生畏期之86物醴重或言午不畲下降，但假设照法正常增重，仍判舄槽重Weight loss of 20-25% (depending on attitude, weight recorded at time of arrival, and age: growing animals may not lose weight, but may not gain normally) or if not measured, characterized by cachexia and wasting syndrome.2.丧失食愁 Inappe

5、tance:小型SWW物完全失食24小日寺或食悠不佳低於正常量之50%建3五日寺大勤物完全失食以或食愁不佳低於正常量之50%Complete anorexia for 24 hours in small rodents, up to 5 days in large animals; partial anorexia (less than 50% of caloric requirement) for 3 days in small rodents, 7 days in large animals.3. 虚弱照法逵食或欺水Weakness/inability to obtain feed or

6、water:勤物在没有麻醉或的状熊下，24小日寺照法站立或桎度勉弓鱼才可站立日寺Inability or extreme reluctance to stand, that persists for 24 hours, assuming that the animal has recovered from anesthesia.4. 垂死/溅死 Moribund state:勤物在没有麻醉或静的状熊下，表?I精神抑半随醴湍通低低於37C日寺Depression coupled with body temperature below 37 ,5. 感染 Infection:照是明可知或因醴温升高

7、白血球数目增加而判舄感染所致 ，且在抗生素治瘵照效亚 伴随勤物全身性不遒病症出现日寺。Infection involving any organ system (either overt, or indicated by increased body temperature or WBC parameters), which fails to respond to antibiotic therapy within an appropriate period of time and is accompanied by systemic signs of illness.6. 出现器官重丧失功能

8、的晦床病症且治瘵照效，或经勤物中心MS判IBS彳爰不佳日寺。如：Signs of severe organ system dysfunction and non-responsive to treatment, or with a poor prognosis as determined by an veterinarian:(1) 呼吸系统：呼吸困I、Respiratory system: dyspnea, cyanosis.(2) 心血管系统大失血、已幻予一次翰液治瘵彳爰仍血(低於 20%)Cardiovascular system: blood loss or anemia.(3) 消化

9、系统：JR重蜓吐或下痢，消化道阻塞，套叠，腹膜炎，内腻摘除手彳有Gastrointestinal system: severe vomiting or diarrhea, obstruction, intussuception; peritonitis, evisceration(immediate euthanasia required).(4) 泌尿道系统：弩衰竭(BUN, creatinine, uroperitoneum的提升)Urogenital system: renal failure characterized by elevated BUN, creatinine or ur

10、operitoneum.(5) 神经系统：中福神经抑制、震H、瘫痪(其中任一肢或以上)、垩寸止痈蜘I治瘵照效之 疼痈Nervous system: CNS depression, seizures, paralysis of one or more extremities; pain unresponsive to analgesic therapy.(6) 肌肉骨骼系统：肌肉受揖或骨折使肢失功能(苫食琪期畿生亚通遏IACUC睿核除外)Musculoskeletal system: muscle damage or fracture resulting in inability to use

11、the limb, unless anticipated as part of the study.(7) 皮唐：照法治疲之分口、重祓性自殛或二级以上之保Integumentary system: Non-healing wounds, repeated self-trauma, second or third degree heating pad burns.7. 月重瘤生成黑册估 Tumorigenesis Endpoints Assessment性或是接椒的月重瘤，均JEfi行估。富勤物身上瘤，每遇 至少检查U次月重瘤生畏情形，U次检查的冏隔不可超遏四天。只要符合下歹0任一项情 沉即需将

12、8&物安槊死。Tumors may occur spontaneously, or be experimentally induced through transplantation or administration of carcinogens. Animals in which tumors are growing should be checked at least two times weekly, at intervals no greater than four days apart. The clinical signs listed below are expected as

13、 part of the experiment and appropriate measures are taken to minimize pain or discomfort in the animals.1) 罩一月重瘤的重量超iSSS物醴重的10%，或是成年小鼠月重瘤平均直彳至超遏20mm,或是成年大鼠月重瘤平均直彳至超遏40mm。A tumor burden greater than 10% body weight, and in an adult mouse, a mean tumor diameter exceeding 20 mm or in an adult rat, a m

14、ean tumor diameter exceeding 40 mm.2) 醴表月重瘤：月重瘤外表出现溃疡、壤死或是感染。Superficial tumors： Tumors that ulcerate, become necrotic or infected.3) 腹腔月重瘤：腹腔巽常攒？I、呼吸困雉。Ascitic tumors - abdominal distension, dyspnea.4) 瘤：神经病症。Intracranial tumors - neurological signs勤物安桀死方法 Method of euthanasia of animals at the end

15、 of study表1. 物安槊死方法方法寸裹孕13日胚胎01-06日窗令07-14日窗令200g时二氧化碳00VVV1. 使用高摩桶装二氧化碳2. 斡行日寺，俺可能在物伺餐房的非公 埸所it行3. 14日窗令以下勤物，不建使用二氧化 碳安槊死，需配合SfHHalothaneMethoxyfluraneIsofluraneEnflurane00VVV1. 具彳肴汽化器等暮用 W,业段言十合逋的 可透祸性密容器以易於麒察勤物2. 大局部的吸入性有害，需在通 凰良好埸所彰1行SevofluraneDesflurane3. 麻醉期勤物可能出现挣扎及焦4. 7公斤以上不建tg使用此方法。Pentoba

16、rbital 100-150mg/kg.IP, IV00VVV1. JS於第三级管制桑品，人iOS先向衡生署申言青使用言午可2. 人有保定勤物及注射的技彳何麻醉彳爰放血000VV1. 放血不完整或心IW采血不完整日寺，勤物可能畲甦醒2. 人有相技彳有麻醉VVVVV1. 人有相技彳有2. 仔鼠需以利的外科剪刀其他HU使 用II商台低温麻醉彳爰fVV000将仔鼠放置檬本杯非petri dish ,浸入冰聚中%勺二十分蚤童，以孑成少胸部活性及流血麻醉彳爰5椎脱 臼000V3人有相技彳有麻醉彳爰注射KCl.2meq/kg, IV000VV人有青期辰注射的技彳何清醒中勤物fVVddd1. 人有相技彳有2

17、. 仔鼠需以利的外科剪刀其他使用台清醒中勤物椎脱臼000dd人有相技彳有乙醍00ddd乙醍具有易燃、易爆的性11,封呼吸到黏膜 刺激性弓鱼，如因10食需求而使用乙B, 1需 全程在抽操作，勤物ftut需置於防爆 冰卧V:推蘑使用的方法。:不推蘑使用的方法。3：物管理委H畲同意彳爰使用的方法。ip：腹腔注射。IV： IM辰注射。表.2 100% CO2安槊死日寺冏参考榇卒Age of miceCO2 exposure TimeRemark0-6 days60 mins一般需配合SfSI法合饼使用7-13 days20 mins一般需配合SfSI法合饼使用14-20 days10 minsw 21

18、 days5 mins来源：Comp Med 55(3), 275-281, 20051.阈防酱孥院勤物申育青表附件 H:WM中86物安槊死日制?及卒JW2. AVMA Guidelines on Euthanasia.2007.American Veterinary medical Association.3. Euthanasia of Reptiles in New Zealand: Current Issues and Methods. B.D.(Brett) Gartrell, E.J.(Ted) Kirk. kokako (2005) 12(1), 12-15.4. Guideli

19、nes for tumor inoculation in rodents. ( :/orsp.rutgers.edu/animals/downloads/ SOPsZTumor_Endpoints_Guidelines.pdf5. :/oacu.od.nih.gov/ARAC/endpoints.pdf. Guidelines for endpoints in animal study proposals.6. :/ ahc.umn.edu/rar/euthanasia.html7. Institutional Animal Care and Use Committee 2nd Edition

20、 2002, provided by the OLAW office of NIH.8. NIH Intramural Research Program s Guideline on Fetal and Neonatal Mouse and Rat Euthanasia.9. Recommendation for Euthanasia of Experimental Animals: Part 1. Working Party Report. Laboratory Animals (1996) 30, 293-316.10. Recommendation for Euthanasia of Experimental Animals: Part 2. Working Party Report. Laboratory Animals (1997) 31, 1-32.11. Review of Schedule 1 of the Animals (Scientific Procedures) Act 1986. Appropriate methods of Humane Killing. Animal Procedure Committee. 2006.