动物采精方法

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1、Sem ltinTre are thrmony-used techniqu forcolletng emen：use o an aiicil vagn,digital mipuaion,a eectejaculation.Tetchniqe uddeeote sces bingllected and the diposion f the individualmal.The AtiicialVaginaticalvaginas(AVs to theiniiae)arused o cllectsmerom may secies，most rmnenty clandhoses，but as shep

2、,goats,rabbits an evencat.Peeuisie to use f A are hat themal e consious,notsigncatly frghteed of people,and more inteted inejcatgthan in kllng huasAnAuses thermaad mchanicalstimlainto sulteejaultion.As switeimg to theigh，AV omposedof ube ithan uter ruerining tat ill howter，inowhichis plced n ineinr

3、that is icted ut riorto ue.The oute liner is filedand ressurizd somehat wit ater t-48egree Celis Showbelow ar AVs egned f use ith ulls(bottm)and rabbits(top)The pprau in h mide a ye f V,usally call diectr con,whch isusd to olle smen omogs.clecsemen,the males lloeto moun and t penis is dvertd ito te

4、AV where he jaculates.Iisbet to allothemale to thrust ito therathe an tring tslietheVoo thealspni.Whatthmale mout dpeds his spies nd temperment.Feas an be ued,b uless th ar n estrus,they rarely eparticpingi therocess,wiha asl ea to inury ofan an al of thoseoled.Anter prblem with usn femla a easrs ha

5、t ifyouiswt th A,theemal oldbebreorpssiy transmi vnerea isease toh male.or colleting semen frm bulls,a train sei usuly ued In te ae ofstllios，patom（dmy)is a popul choice fo a moun.git Maipultionay mscan b inuced o ejulate by appling pessre an masage tohepenis.Afrhe maebcomearsed,a diec cone fsome yp

6、e,attched cllecon tube,is ipped over th peniso acilitat harveing te seen.T ehque is cmmly s fo oleting semen from pigs and dogs n itial trainin prodan ehelpful,and aig female n etrs taroue the maoften is ueul,partiulrwhe the mle is h.Aothe typ f ssage usdocasioll wth bulls to srkethe ampllae(termial

7、 v dferens),em veles dprostae la through thwal of th etm；in almst allcs，the ll ust b resaindstaning inaute and sate o allow relaxainand extru the pens.In conjunction withaurethatter,one can obta a terlealaeing this teniueemen iscolectedroutiely fomcickens an trkeys uing form ofdital mpatn.Eleroejuat

8、ionElectoejculati nvolvesapplinaseris f shr,lw-vltge pses ofcurren othepelcnres whic arenvolvdin theejaculatry espose tsou like a etemeyunpleasntexperiec,ut doesnt seem to as much distrs in buls(althoughtheydo need t bscurely rstine），an icoducdunder antesia in mn pecies.lectrejaculation an sed ith l

9、most any mammal.Withfew exeptions,eecroeacuaio is he l chniquusful frcoeti semen from ild aimals，in wich casehemal isastetzed rir to he produrenoteradvatagof elctroejaclaton is that t doesnot quire mount animal,ancn be apled in the ield uinga attr-poeredunitlly,electroejautiocbe ue t obainsemen frma

10、imals tht are phial incabbl of ountg dueto sculoseleal dseaeor injury.i ed r collectig semen fm uadraplgic r paraelegi men whdesira chd bu cnot eaculaeduetspin crd injr.Wt bull,whre hee is bundant experienc colltngseen yboth rici vaina ad elecojaculain，the samplscolltedusing a lctrjculat uually have

11、alarger vole(du to excesie aessory ganscreion）an a lowe number ofspem.hree pieces f eupmen ae required for ltrojacaion.Th ectroeauaoritself is a powe py wth rheotas tocotrol te apltu o deliverd rre and lts of circitr topreent acienal eectrocto.econd,oe es a oeco tube，usallattachd to a latx rubr cone

12、(lovin cup）n whic to cole thee.Fialy,pulse of crrent apied rough anelctrejcatr prbe.h rob i nsered int the recumschtha th electrods ie ihin pelvi caviy.Older robes hcircuar elecodes,which often case unesirblmuslecntctions;robwih pralle electrdes one idmiimz this poblem Three prob r hwn be-the uppeon

13、eis a comria produt fobuls an t ler probeswerehanda fr ue with sall leopards(dl)andferet（botto)Succssful eecrojaculainoananmlemands sll.It isn simply amate ofunchin ttos ad trning kno,u reqis fineendermning the oer tmng and amplitd of pussto app to a gien le.Seme Collectifrom Blls large majoritydair

14、 owsin heS,anda anEuropea couris ae artificiall insemnated.Arificil iemnation fbefcows isalo pour,paticularly inpurebred hrs.Teavantage of A vernaurlserieis thatit fcitates apid gentc imrovemnt by alwing use ofony te topbls.ome ulls avesiredmr an 1,000 ofpin AI.eme imost conly collectefom bulsn bul

15、studsuinanartificl vgin,a decibd belowElectroejaculaton sanenativ metuewi bullstat cnnot mounare oo racous forea adig（e.g.angeblls)inlly,sencan becollcted b ssage of he sei vclesand mpulaeerectumSeen Coltio via Arificia Vagiaemen colein from ull uig an Arequire thre peope:e tohndle the tese nima,one

16、 to otolte bul and ne ole the eme I is prtanhat te collcton area hve nn-slipflooring t avoid iure and because ejacaton may be inibiteifhebuis evou about his footing Bulls ar heavy and shoulhave ularhof ar.Poor oof condin cannhibit the bull rom montingo cauep whn smoting.A teer i ostcommnly used asat

17、ease and mt ilemaleteaser arnot reomnedbecause f th potntial risk f intrssion nd spreadi of neeldisese.Theteas houd be acalmanialof the propriate sifr th ull beig clected.The ac andrear qatef thteasare ashed th diinfcnercolleo dayIt ao commo frteer uarersto be cipp rotinl.Tlls prputial hair should b

18、 ldin pepationo usigthe.s anitayprecautons intende to mnimizemcrbial ontaminto of he sme being colec.Fr colletin,the tesersposioned stright n rot of theulfr munting.feis,ihelpsto arose hebu ifth ster ised aoud thecollction rena hth ull beh,then spedabrupl,similart te beaior f a cow n estuse ariicalv

19、aginal（ictuedt right）uss therml and canical stimulationto smut ejaculatio.Thliner f t AV is fild ith water at 4-4 dgees Ciu，and th inne urfae is bicate withsothng lke K jly.n inatng cone aced oe th ed from whih te colectiontube protud tavoid subjecin thesemen to eperaue shock.False mouig is an ffcti

20、v wy t sexualy stmuathbull.Proving tfale ounts with t minutof ctive rsan and ne adiional fse mount mimizesspm cl numers.Fia pptios ar made to h Vbtween the secn and hdfals onts per costuction f th i import toiddamang e bls penis adto aod sressig sperm es.uccesfl see collection th n AVepns on thebllb

21、eingcomfortable round eole，an eyned berane t sethe AV.over,ulls r big ad dngerous,a pesonelftyould b mpsizdconstnty.Most bull in rtcal inseinatio ceners ve anse ng nsalled as a auable ad umaeans of pyicalcontro Bullsnergoingemolection shoudbe haltered and oneshold nevereaull uy thei se ring-ifstarle

22、d,ten can rip i utwhich s not nly quite ramat uildsn aima that my beecepioy diffit to ontol.Dingcoletin,th pesohandln teAustreanawareof where heir fee are eaive to t ull.s te nimal ecuates,itis ommn frh to jum fow.Tavoioot ury,oletos shouldwear boots with steeltoes.I arifiialnmintin eners,uls ae ypc

23、allycollct2otime per wee，th or ejaculatespe colectio day.Thefowin imas depictroutineemencllctn rm a ull.False munin to rousebulArec enis ndictesusaColetr ady with Clector divrtin penist the AVSeme valuaioSmenhuldbealuated rsly for anoml appearane.hepsenceof sallls or bood ca idcte sh cnitos sseminav

24、esiclisParmteomal Valuaculate olme ml（rang 1-15 ml)Sem conntraton1 milionml(ange300-250 milonl)TtalsrmperejculaeTpaly 4-illionPgressiv motilitGe thn 0%MorhoyGear hn70%normalSen Clection fro ogsmen is coleced ro ogs or breedingsones xa,a wll as for rtifcia iemition.Semen ectefo nsemnaion cnbe ud fres

25、,o can b ooled ad hippd to anotlocatio.Cnine sen cn als berozen，allowin ng tem storge.Aoteindication fr cllectin smen iso oban prostic fluid for ctre r ctolgy in cse o suspecteprostaic dee.emnColeiSemen an e ollectedfrm most maleswithoutte need r ateseitc,particulaly if the ale has had emen cocted p

26、reviosly.Howeer,use o bith llmostertaily xpdie h pocedue adaloore sperm o be harsed.Idealthe teasershould benestrus，but consierng h lengf he cain yle，that oftn dficult to rrane,and ridly,nn-eusbitch ill often eve he purse.I bich is used,sheuld ontrlld wth heea qarters cinh male.Anaenaie ean of stimu

27、latgt mal i t peent hwia agal swabfom anetous itch-or ovniec,aumber of schswbs c be preaedfrm estrous ch andstored rozen until ededCanine semen ollectedusindigialpressure and masage.ostailures arie becaus theml i shy oroteientmidaed.It elps to perfomhecollection on a onslip surfae sucsa carpet.f he

28、l appas eros orths ish fisttie,a taer bith ma hlp ondby.emen is coeced ihot allowig the mle to ou.Altexcolcton onewith an ttached b i ommn d.san atratie,ome peoplepreferto use a disosale bybotle linr Both ollcontols re shownClectingsemen from dosis no ficult,ut iemayhins,ismuch esier aftryoue done i

29、t a timeor to.he asicrocess is cduced n tefollwn series of steps:Grasp tprepu and pul/sh t ak to expose t t ofthe peniide the coection cone ove heprotuing penis andide iover th penis,pusin the ppucebakv o t buls gans(see images eo).Lock youringe in arngaoun the peis,ssentily hldingth blis ndiinide o

30、ur istpply presueith foard andbackr memnt;in mos ass,e mal i bgn ust bak and forth.Wa for semen to flow in tcllection tub.Mstogs stp hrustg the bin toejaculate.Ctinue to applypresure nil yu obsrve a rystal cear flid（pocfld)begi o flw into the colectin ue;atht time ocan gnl side th oletion ceoff th p

31、isRetractionf te preuceolecon coneoand pesuebeingapleAfer collction te theengored bulbu glandisA rathe ommon problem encounterd n collecing canin smen is thatthemale vlops aneretioprior to bein le t extend he peni ad bubus glndisout f he epuce.Semen can b lced in ths mnnr,bu ot as easily.Ifhshappen，

32、imply tak he male aisnceawy to ethim alm own,then try agn.SeenEvauationDgs ejalate smen asthree dstincv fration.By salelstc clletion tube,theliverothe three frtions eiy moitoePrspermfraton:this is suy lghty couyin peraceands a volume o rohly 0.to m.Sperm-rich frctin:n rmal dogs,this racion is distin

33、ctly iky iaearancProstatc fraction：ths cin is recognzed as a cralclearfluid flowig int h ue,nd ejaculated slowig over aprlonged priod of ie.In mos ss,lletion is toe a son ashis frcion is ognzed.Nomlanges for seminl haraceritic indgsare descbediheolowng tbe:aramerNoralValsjcult vlum(wtho static fraci

34、n）1-6mprm oncntraio100-50milon/motlsperm er jaculte30-mlProgressive moilityGreter tn70%Morpholgyreate an 80%ormalSemen Colcion omtallionsemenis collecte from stallnas art ofbedngondnessexam,a wl as or rticil insinaton.emen coleced frinsemination can be used res,cooledndhpped nohe locatio,o sted frze

35、 r se in he future.Use ofan rtifial vagin is arand a the most mmo ethodforolcting eme fom salions The A ued ih stllios simlatothat usd for peies uchas bulls，xet larr.The mge bel shows ppula dign fa stallion A（diasembled）.Due tothe lrge juate olum,collctiobttle isused inseadof tue.he blue cnenextt th

36、e ot a spei hat worby thesemenclleo;rather it is an nsltingco tt placdove he end ofhe V tosild thsemen colleio bottle fro theral sock.Sllin eme fte contins a arge volum gel,gelatinssecretonrm te seminalveice.Gel is ejaculatd afte tsperm rch factn o nimzcontminationof heeme hhs gl,a non flter anbe in

37、sted into the olecon bttle hen th Ai asmble(shon abov）;te gel trape in thefil andiscaded.rtain cmbinations of taquilizs have been used duejaultonrom stallion Thistechniqe is usefur collectig semfromstalli hat canot mot mareor phantom e to musloelaldisese Elecroejaulationuldnobe atemt ith stallio.Sem

38、enCllecionens fnclcted from tallions sing a estrou or ovriomied aer re.The dsadvantages of thi prc e tht it ca bedneusforthe lletio tea,the me ad the tallio，ad there sgod sbliy hat someis th mare ill b bredb the stallin.T protec thetallon,th meshoul bhobbldt pevent kckin.An alternativet ustease are

39、is to e hntom mount-inanimate mre looka-like Roughly2 of sions raine t mount and be collete using a patom.This mtho is ouied owWhen a antm usd for en coetio it is tl uselto have a live,estros mare to tese and arosethestalin.Such ateasr arcn b een i thpicr at ight stndingin a stonet to theantomhe img

40、e below show ayc series o et incoletg ee from tallionotethat al thinvoledpepeare wing heles.Stallonsrlarg，agressive,rdictle and dangros.Theyreqntly stike,kik and bite.Hence，prsnnel ty soud e givencnsat atention.Stalvsiing te tease maeWashng th ns tomnimizeotamintioalionrey o moutStliononnColecng sme

41、n ith he AVallonsing noutSem EaluaionAs fr an specis,semn sholbe evauated imeiatel foowing oletion for goss abormlite sch as od.Noavlueadrangs fr tlio sen a prsentd beow.PrameNor auesEaulae olum0ml(rane 30-5)Ser concentration20 milon/l(range30-00 milli/ml)Total perm ejaculeTypially 7-1 bilionProgressv mtilityGae than%MorpholyGreate hn60%normal