温病气分证论文：白虎汤治疗内毒素致家兔温病气分证的实验研究

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1、温病气分证论文：白虎汤治疗内毒素致家兔温病气分证的实验研究【中文摘要】以家兔为实验动物,用大肠杆菌内毒素建立温病气分证动物模型,通过经典药方白虎汤进行治疗和验证,观察家兔的体温变化和临床表现,对血清中细胞因子水平、生化指标、免疫学和抗氧化指标进行检测。为温病气分证动物模型的建立、理论研究以及提高其临床治疗的效果提供一些技术资料支撑。将18只大耳白家兔随机分为3组,每组6只。气分证组,耳缘静脉注射大肠杆菌内毒素15g/kg,灌服生理盐水7 ml/kg。白虎汤组,耳缘静脉注射大肠杆菌内毒素15g/kg,灌服白虎汤7 ml/kg;注射内毒素后6和12 h时再灌服白虎汤两次。对照组,耳缘静脉注射相同剂

2、量生理盐水,灌服生理盐水7 ml/kg。观察各组家兔体温、症状表现和体征的变化。对照组和气分证组于注射内毒素2.5 h后心脏采血,采血完毕后处死家兔,对心、肝、脾、肺、肾等主要脏器进行病理学观察,检测各项血清指标。白虎汤组于注射内毒素24 h心脏采血,采血完毕后处死家兔,对主要脏器进行病理学观察,检测各项血清指标。1、注射内毒素后,与气分证组比较,白虎汤组家兔体温升高缓慢,蜷缩、发抖、耸毛、呼吸、心率加快及渴饮等症状明显减轻。对照组一切正常。说明白虎汤可降低气分证家兔的体温,明显改善气分证症状和体征,能够减轻主要脏器病理组织损害。2、气分证组血清IL-2水平显著低于对照组和白虎汤组(P0.05

3、),而IL-4、IL-6、IL-10和IFN-水平显著高于对照组和白虎汤组(P0.05);气分证组TNF-水平极显著高于对照组和白虎汤组(P0.01),对照组显著高于白虎汤组(P0.05)。3、气分证组血清ET-1、ALP和AST的含量显著高于对照组和白虎汤组(P0.05),而血清UA和UREA的含量显著高于对照组(P0.05);气分证组血清ALT的含量极显著高于对照组(P0.01),而血清CREA和TP的含量极显著高于对照组和白虎汤组(P0.01);气分证组血清TC的含量显著低于对照组(P0.05),极显著低于白虎汤组(P0.01)。4、气分证组血清CH50的含量显著高于对照组和白虎汤组(P

4、0.05),而血清IgA的含量显著低于对照组和白虎汤组(P0.05);气分证组血清IgG和IgM的含量显著高于对照组(P0.05)。5、气分证组血清MDA和NO的含量显著高于对照组(P0.05),而T-AOC的含量显著低于对照组(P0.05);气分证组血清SOD极显著低于对照组(P0.01),白虎汤组显著低于对照组(P0.05)。【英文摘要】Rabbit as experimental animals, establish the Qifen syndrome in seasonal febrile disease animal model with Escherichia coli endo

5、toxin, by the classical prescription baihutang to treat and verify it, observed the rabbits temperature changes and clinical manifestation, detection serum cytokine levels, biochemical indicators, indicators of immunology and antioxidant. Provided some technical information support for established Q

6、ifen syndrome in seasonal febrile diseases model, theoretical research and improve the level of clinical treatment.18 rabbits were divided into 3 groups randomly, every group of 6 rabbits. Qifen syndrome group, Escherichia coli endotoxin 15g/kg are injected by rabbit ear vein, fed normal saline 7 ml

7、/kg. Baihutang group, Escherichia coli endotoxin are injected by rabbit ear vein, fed baihutang 7 ml/kg to rabbits; after injected Escherichia coli endotoxin 6 and 12 hours fed baihutang twice. Control group, injected by rabbit ear vein with the same dose physiological saline, fed normal saline 7 ml

8、/kg. Observed the changes of rabbits body temperature, symptoms manifestation and signs. Blood from control group and Qifen syndrome rabbits heart after injected endotoxin 2.5 hours, killed the rabbits, generally pathological observation for heart, liver, spleen, lung, kidney and other major organs,

9、 detection each indicator of serum. Blood from baihutang group rabbits heart after injected endotoxin 24 hours, killed the rabbits, generally pathological observation for major organs, detection each indicator of serum.1. After injected endotoxin, compared with Qifen syndrome group, the baihutang gr

10、oup rabbits body temperature increased slowly, curled up, shivering, towering hair, breathing and heart rate up, drank and other symptoms significantly reduced. The control group all nomal. Showing that the baihutang can reduce the body temperature of rabbits with Qifen syndrome in seasonal febrile

11、disease, significantly improve the Qifen syndrome in seasonal febrile disease symptoms and signs, reduce the pathological damage of major organs.2. Qifen syndrome group, the level of IL-2 in serum was significantly lower than the control group and the baihutang group(P0.05), but the level of IL-4, I

12、L-6, IL-10 and IFN-was significantly higher than the control group and the baihutang group(P0.05); Qifen syndrome group, the level of TNF-was extremely significantly higher than the control group and the baihutang group(P0.01), the control group was significantly higher than the baihutang group (P0.

13、05).3. Qifen syndrome group, the content of ET-1, ALP and AST in serum was significantly higher than the control group and the baihutang group(P0.05), but the content of UA and UREA in serum was significantly higher than the control group(P0.05); Qifen syndrome group, the content of ALT in serum was

14、 extremely significantly higher than the control group(P0.01),but the content of CREA and TP in serum was extremely significantly higher than the control group and baihutang group(P0.01); Qifen syndrome group, the content of TC was significantly lower than the control group(P0.05), extremely signifi

15、cantly lower than the baihutang group(P0.01).4. Qifen syndrome group, the content of CH50 in serum was significantly higher than the control group and the baihutang group(P0.05), but the content of IgA in serum was significantly lower than the control group and the baihutang group (P0.05); Qifen syn

16、drome group, the content of IgG and IgM in serum was significantly higher than the control group(P0.05).5. Qifen syndrome group, the content of MDA and NO in serum was significantly higher than the control group(P0.05), but the content of T-AOC in serum was significantly lower than the control group

17、 (P0.05); Qifen syndrome group, the content of SOD in serum was extremely significantly lower than the control group(P0.01), baihutang group was significantly lower than the control group (P0.05).【关键词】温病气分证 内毒素 白虎汤 家兔【英文关键词】Qifen syndrome in seasonal febrile disease Endotoxin Baihutang Rabbits【目录】白虎

18、汤治疗内毒素致家兔温病气分证的实验研究摘要2-3Summary3-4第一章 白虎汤治疗温病气分证的研究进展9-261 温病气分证及其动物模型的建立9-151.1 温病气分证及其研究状况9-131.1.1 温病气分证9-101.1.2 气分证阶段主要的病理变化10-111.1.3 气分证阶段的免疫相关性探讨111.1.4 温病气分证的防治11-131.2 温病气分证动物模型建立的研究13-152 内毒素及其温病气分证动物模型15-192.1 内毒素及其化学组成15-162.2 内毒素的生物学活性162.3 内毒素的致病机理16-182.3.1 内毒素诱导细胞因子的合成与分泌17-182.3.2

19、过敏性毒素182.3.3 血小板活化因子182.4 内毒素建立温病气分证的研究18-193 白虎汤及其现代药理临床应用19-263.1 白虎汤之历代评述19-213.1.1 各家方论集锦19-203.1.2 名家医案20-213.2 白虎汤的药理研究21-233.2.1 君药石膏清热机理21-223.2.2 知母作为臣药的清热机理223.2.3 石膏和知母的合用疗效22-233.2.4 白虎汤增强免疫及抗感染作用233.3 白虎汤临床应用和研究23-243.4 白虎汤治疗温病气分证的研究24-26第二章 白虎汤治疗内毒素致家兔温病气分证的实验研究26-441 材料与方法26-281.1 材料2

20、6-271.1.1 实验动物261.1.2 试剂及药品261.1.3 仪器设备26-271.1.4 中药白虎汤汤剂271.2 方法27-281.2.1 气分证模型建立27-281.2.2 血液标本的采集、处理及各项指标测定281.2.3 统计学分析282 结果28-322.1 体征变化28-292.1.1 症状表现与体征变化282.1.2 体温变化28-292.2 病理学观察29-302.3 血清指标测定30-322.3.1 血清细胞因子水平302.3.2 血清生化指标30-312.3.3 血清免疫学指标312.3.4 血清抗氧化指标31-323 讨论32-423.1 白虎汤对家兔气分证症状表现及体征的影响323.2 白虎汤对气分证家兔血清细胞因子水平的影响32-343.3 白虎汤对气分证家兔血清生化指标的影响34-373.4 白虎汤对气分证家兔血清免疫学指标的影响37-403.5 白虎汤对气分证家兔血清抗氧化指标的影响40-424 结论42-44参考文献44-49致谢49-50个人简介50-51导师简介51-52