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1、SB产卵板繁殖期，IV期卵巢放入隔板及斑馬魚上盒與普通下盒組裝上盒底以網狀材料代替123490%LeibovitzL-15，pH990%LeibovitzL-15Leibovitzs MediumspecificallydesignedtogrowncellsinaCO2freeatmosphere.Thestandardsodiumbicarbonate/CO2bufferingsystemisreplacedbycombinationoffreebasicaminoacids,phosphatebuffersandhigherlevelsofgalactoseandsodiumpyruva
2、te.Asaresult,themediumdoesnotrequiresupplementationwithsodiumbicarbonateandcanbeusedunderconditionsoffreegaseousexchangewiththeatmosphere.ThemediumcanbeusedtogrowhumantumorcellsandembryoniccellsandalsoestablishedcelllineslikeHeLaandHep-2.Themediumisfrequentlyusedindiagnosticvirologywheretissuecellli
3、nesorstrainsneedtobegrowninclosedsystems.Leibovitzsmediumobviatestheneedoffrequentmediumchange.Composition:nIngredientsmg/LnINORGANIC SALTSnCalciumchloridedihydrate185.000nMagnesiumchloridehexahydrate200.000nMagnesiumsulphateanhydrous97.720nPotassiumchloride400.000nPotassiumphosphate,monobasic60.000
4、nSodiumchloride8000.000nSodiumphosphate,dibasicanhydrous190.120nAMINO ACIDSnDL-Alphaalanine450.000nGlycine200.000nL-Arginine(freebase)500.000nL-Asparagine250.000nL-Cysteine(freebase)120.000nL-Glutamine300.000nL-Histidine(freebase)250.000nL-Isoleucine250.000nL-Leucine125.000nL-Lysinehydrochloride94.0
5、00nL-Methionine75.000nL-Phenylalanine125.000nL-Serine200.000nL-Threonine300.000nL-Tryptophan20.000nL-TyrosineDisodiumSalt276.160nL-Valine100.000nVITAMINSnCholinechloride1.000nD-Ca-Pantothenate1.000nFolicacid1.000nNicotinamide1.000nPyridoxinehydrochloride1.000nRiboflavin-5-phosphate,Na0.100nThiaminem
6、onophosphate1.000ni-Inositol2.000nOTHERSnD-Galactose900.000nPhenolredSodiumSalt11.000nSodiumpyruvate550.000nDirections:n1.Suspend14.0gmsin900mltissueculturegradewaterwithconstant,gentlestirringuntilthepowderiscompletelyndissolved.Donotheatthewater.Note:Presenceofslighthazinessinthemediumisduetoinher
7、entnatureofsomeofntheingredientsinthecomposition.However,thiswillnotaffecttheperformanceofthemedium.n2.AdjustthepHto0.2-0.3pHunitsbelowthedesiredpHusing1NHClor1NNaOHsincethepHtendstoriseduringnfiltration.n3.Makeupthefinalvolumeto1000mlwithtissueculturegradewater.n4.Sterilizethemediumimmediatelybyfilteringthroughasterilemembranefilterwithaporosityof0.22micronorless,usingpositivepressureratherthanvacuumtominimizethelossofcarbondioxide.
斑马鱼的性腺发育(10238473139)
