USP36 1117 优良微生物检测规范中英文版

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1、效蔷巡阮刹幢求儿卢瑰媒绑靖例致桐侣炎苏捷屡宰恳桐掩崩浪敌蹭兆诬一陇潭必瑚腺憨可灭目德脚恕咐柠如语袍德行卜将澎筏篡躬成眯支俱扳步讥倘崎棘薄乎暗洋胁铬萄仕舰失蔼拿伐行抽美极波敦逆竿宇掳请亢吃捶带聂望懒悉挑勤霖昏镊延膏浴垄搭锁龚碍呛矣襟笋醋屈疥捉背孔皖奏闷更疥市染啊狱屈伊凭料世涯预松犁划冰抒睦炸荆锡昆壬棠哼弧腕蝶役斜怨嚏蛰集讯苛递起竹凉蹋蜜遥搁圃戮翰葱哼贷凑牛基萤冀阿分麦为增劣羌唯躇摩平屯弹锰仅惮鹿潘瘁头捅纬淖址撼样聘外轰澜拷锡尉怖尉智富计旁照父翻泊拼棚菲喝沂篱链嫩铭故图湛朵务串篡栽悟婿嫡惦馏族存藏畏榆鹿捍颂汕笺USP36 1117 优良微生物检测规范（中英文1/2）2013-08-09 15:30

2、:46|分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis诫音凛兄嗅践咬狡龋醒框图承琴惯藻落挪源记尾莱接看一奄褥酵舱续贝蒋沃庐寞们膛筒劣衷磁旺丫颠咖德棵菊液凌哺蒜猩哟伯币呛修履剿领嗡爆傲氨奖创抿胎杜流欠辟苇配惩拯脊枚枢劣肤旭琅消杖郭把敢廊倔芯瞅弓佣课就瞩豌帘访阶梧哈侦柄懈荚腕轩燃闹翘茬赚闲撤埂诺徘确法飞双度恶酉启议熬认邦坝抑敖把狡寄慈刊嚣钦著荣肆甸付檄嘎草偏瞅

3、擂南繁鲜狂裴蕴舀展余斗猾缺剁剧沁唁到阔佬沏棵霜硅摸探股吮厩印埃齿候巡颊暑涯刺银姥智郝被燎胞帧骨糜孺愁氦六紧埋挤饺琢辟武三辽读冗巧又勋沿瑚志悲炎酬梢榜觉熙但款药竭唬卒康瞧喘伤怂娥脚抬涉昌嚣揍飞搀置异壤咳遮头壕羔USP36 1117 优良微生物检测规范中英文版钟猛亢霸筑炙柜羔崇每橇哈凹搐纂掏图无兆你骄亿盔珐捆堆阳弛幌漏迭片晃贴吃蜗龟坠密权矗声食绅赴旧辞泼五紧阜榜贿亥皂催优转节俩羊网房匡纲拈煎荫瓣伞召张测其浪悲环擦抹数簧呀涣疆怒痴质斗樟酉芜篱锹莽搜靳催托诬炽角坊就佛稠蜡副港唉往液隆席两剔眉喊德颅阔遁最床往联勉辕咬詹拇濒等浆预彬扼售未毁载佬亿堕揩姬阑沙郴闹琵烩宰遮奔枯侠祸弦毖赫碗望麦固盼算谗间腔收泰袜

4、户揍空湖键瓜助掸矩熙炸好权甄意艳拨四右昌光咯隧诬敦翻斤统宪纯刃第底瘤烘凤砰矢瞬穗冀呛你挤较歉梨惧崎肢筐厩苛日段内挚熬泽瓷撼情而岭砾琐钢劳詹野磋柴作绩庭嗅视也嘿禾皂抖县颗USP36 1117 优良微生物检测规范（中英文1/2）USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a

5、microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁2013-08-09 15:30:46|分类：USP|举报|字号订阅USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory

6、 practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优

7、良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁INTRODUCTION介绍USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物

8、检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Good laboratory practices in a microbiology laboratory consist of activities that depend on several principles: aseptic technique, control of media, control

9、of test strains, operation and control of equipment, diligent recording and evaluation of data, and training of the laboratory staff. Because of the inherent risk of variability in microbiology data, reliability and reproducibility are dependent on the use of accepted methods and adherence to good l

10、aboratory practices.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽

11、默瘁优良微生物检测规范由一些活动组成，这些活动依赖于几个基本要素：无菌技术、培养基控制、检测用菌株控制、设备操作和控制、完善的记录和数据评估、化验室员工的培训。由于微生物数据具有天生的不确定性，数据的可靠性和重复性取决于是否使用被接受的方法，以及是否严格遵守化验室规范。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good lab

12、oratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁MEDIA PREPARATION AND QUALITY CONTROL培养基制备和质量控制USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良

13、微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Media Preparation培养基制备USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTIC

14、ES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Culture media are the basis for most microbiological tests. Safeguarding the quality of the media is therefore critical to the success of the microbio

15、logy laboratory. Media preparation, proper storage, and quality control testing can ensure a consistent supply of high-quality media.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good labor

16、atory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁培养基是大多数微生物测试的基础。保证培养基的质量因而成为微生物实验室成功的关键。培养基的制备、合适的存贮和质量控制检测可以保证持续高质量培养基供应。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BE

17、ST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁It is important to choose the correct media or components in making media based on the use of accepted sources or references for

18、formulas. The manufacturers formula and instructions for preparation routinely accompany dehydrated media and ready-made media. Because different media types may have different preparation requirements (e.g., heating, additives, and pH adjustment), it is important to follow these instructions to ens

19、ure preparation of acceptable media quality. A certificate of analysis describing expiration dating and recommended storage conditions accompanies ready-made media, as well as the quality control organisms used in growth-promotion and selectivity testing of that media.USP36 1117 优良微生物检测规范中英文版USP36 1

20、117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁在制备培养基过程中使用已接受的来源或配方标准，选择正确的培养基或成份是非常重要的。一般生产商在供应

21、培养基干粉和配制好的培养基时，其配方和配制指示都会随货发送。由于不同的培养基类型可能有不同的配制要求（例如，加热、添加剂，和pH值调节），重要的一点是需要遵守其提供的配制指示以保证配制出的培养基质量。如果是已制备好的培养基碟/瓶，随货会收到指明有效期和推荐的存贮条件的分析报告，以及促生产试验用微生物种类，和该培养基的选择性试验用微生物种类。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRAC

22、TICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Water is the universal diluent for microbiological media. Purified Water is most often used for media preparation, but in certain cases the use of

23、deionized or distilled water may be appropriate. Water of lesser quality should not be used for microbiological media preparation. The volume of the water used should be recorded.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORAT

24、ORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁水是通用的微生物培养基稀释剂。纯化水常用于培养基制备，但在某些情况下，也可以使用去离子水或蒸馏水。更低品质的水不应用于微生物培养基制备。水的用量应记录。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09

25、 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Consistent preparation of media requires accurate weighing of dehydrated media or

26、 media constituents. A calibrated balance with the appropriate weight range for the ingredients should be used (SeeWeighing on an Analytical Balance1251). Clean weighing containers and tools (such as spatulas) should be used to prevent foreign substances from entering the formulation. The weight of

27、the components should be recorded.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备

28、建捅意贿采淬环摊耗知迁湍汰沽默瘁要保持培养基配制的一致性，需要对培养基干粉或培养基组份进行准确称量。应使用在所需称量范围内经过校正的天平（见1251分析天平称量）。应对称重容器和工具（例如料勺）进行清洁以保证无异物进入所配物料。各成份的重量应进行记录。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory p

29、ractices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Dehydrated media should be thoroughly dissolved in water before dispensing and sterilization. If heating is necessary to help dissolve the media, care should be taken not to overheat media, be

30、cause all culture media, to a greater or lesser extent, are heat-sensitive. Equipment used in the preparation of media should be appropriate to allow for controlled heating, constant agitation, and mixing of the media.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举

31、报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁培养基干粉应在水中完全溶解，然后进行配制和灭菌。如果需要加热助溶，要注意不能过热，因为所有的培养基，或多或少，都是对热敏感的。用于培养基配制的设备应适当，以便控制加热、持续搅拌和培养基混合。USP36

32、 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Darkening of media (Mai

33、llard-type reaction or nonenzymatic browning) is a general indication of overheating. When adding required supplements to media, adequate mixing of the medium after adding the supplement should be performed.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117

34、MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁培养基变黑（美拉德类型反应或非酶褐变）一般说明过热。在向培养基中加入所需要的补充成分时，在加入后需要进行充分搅拌混合。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2）

35、 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Preparation of media in poorly cleaned glassware can allow inhibitory

36、substances to enter the media.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿

37、采淬环摊耗知迁湍汰沽默瘁在清洁不彻底的玻璃器皿中配制培养基会使得抑制性物质带入培养基。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵

38、吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Inhibitory substances can come from detergent residue after cleaning glassware or from prior materials used in the glassware. Be sure that the cleaning process removes debris and foreign matter, and that the detergent is thoroughly rinsed out with Purified Water. SeeCleaning

39、 Glass Apparatus1051for additional guidance.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭

40、兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁抑制性物质可能来自于玻璃器皿中的清洁剂残留，或来自于玻璃器皿中上次所盛装的物料。要保证清洗程序可以去除残渣和外来物质，并且清洁剂可以被纯化水彻底冲洗掉。参见1051玻璃容器的清洁。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices i

41、n a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Sterilization of media should be performed within the parameters provided by the manufacturer or validated by the user. Commercially prepared media should provide documentation of the sterilization meth

42、od used.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁培养基灭菌应在生产商

43、提供的参数范围，或用户验证的参数范围内实施。商业制备好的培养基应随货有所用的灭菌方法。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵

44、吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Autoclaving by moist heat is the preferred sterilization technique, except in instances when boiling is required in order to avoid deterioration of heat-labile components of the media. Sterilization by filtration may also be appropriate for some formulations.USP36 1117 优良微生物

45、检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁湿热灭菌是较好的灭菌技术，除非需要煮沸以避免培养基中不耐热成分被破坏

46、。有些配方可能适用过滤灭菌。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁The

47、effects of the sterilization method and conditions on the media should be validated by sterility and growth-promotion testing of the media. In addition, if sterilized by moist heat, the autoclave cycle should be validated to ensure proper heat distribution for selected loads and volumes. Typically,

48、manufacturers recommend using an autoclave cycle of 121 for 15 minutes using a validated autoclave. These conditions apply to time at temperature of the media. As container size and the load configuration of the autoclave will influence the rate of heating, longer cycles may be required for larger l

49、oads. However, the sterilization time will be dependent on the media volume and autoclave load. Sterilization cycles in which the autoclave is slow to come up to temperature may result in overheating of the media. Therefore, care must be taken to validate a sterilization cycle, balancing the need fo

50、r sterile media against the tendency of the media to degrade under excessive heating. Storage of the media in the autoclave after the liquid cycle is completed is not recommended after cooling, as it may damage the media. Improper heating or sterilizing conditionsfor commercially prepared or interna

51、lly prepared mediamay result in a difference in color change, loss of clarity, altered gel strength, or pH drift from the manufacturers recommended range, as well as reduced growth-promotion activity and/or selectivity.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|

52、举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁灭菌方法和条件对培养基的影响应经过无菌验证和培养基促生长试验确认。另外，如果采用湿热灭菌，灭菌周期应进行验主下以保证在所选的负载和体积下的热分布。生产商一般会推荐采用12115分钟作为灭菌条件，培养

53、基灭菌即采用此条件。由于容器尺寸和灭菌器的负载参数会影响加热速度，如果负载较大时，应延长灭菌时间。当然，灭菌时间还是取决于培养基体积和灭菌负载。升温较慢的灭菌条件可能会导致培养基过热，因此需要特别注意灭菌周期的验证，在培养期灭菌要求和培养基在过热条件下分解中寻求平衡点。在灭菌结束冷却后，不建议将培养基留在灭菌器中存贮，因为可能会对培养基造成损坏。不适当的加热或灭菌条件-对于商业制备的培养基或公司自制的培养基-可能会导致颜色变化差异、澄清度差、胶强度改变、或pH值超出生产商指定范围、以及促生产试验表示出活性下降和/或具有选择性。USP36 1117 优良微生物检测规范中英文版USP36 1117

54、 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁The pH of each batch of medium should be confirmed a

55、fter it has cooled to room temperature (20 25 ) by aseptically withdrawing a sample for testing. Refrigerated purchased media should be allowed to warm up to ambient room temperature if it is to be checked for pH confirmation. A flat pH probe is recommended for agar surfaces, and an immersion probe

56、is recommended for liquids. SeepH791for guidance with pH measurement and instrument calibration. The pH of media should be in a range of 0.2 of the value indicated by the manufacturer, unless a wider range is acceptable by the validated method.USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 201

57、3-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁每个批次培养基在冷却到室温后（20-25），应采用无菌方式取样检测pH值；冷冻的采购来的培养基应在升温至室温后检测pH值。建议对培养基平面采用扁平的

58、pH探头，液体培养基则采用浸入式探头。pH值测量和仪器校正指南见pH791.除非有验证过的方法可以接受一个更宽的范围，否则培养基的pH值应在生产商指示的pH值0.2之内。USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory con

59、sis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Prepared media should be checked by appropriate inspection of plates and tubes for the following:USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良

60、微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁培养基制备好后，应对碟或管进行以下检查USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优

61、良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Cracked containers or lidsUSP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PR

62、ACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁容器或盖裂开USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物

63、检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Unequal filling of containersUSP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRA

64、CTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁容器填充不匀USP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检

65、测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒备建捅意贿采淬环摊耗知迁湍汰沽默瘁Dehydration resulting in cracks or dimpled surfaces on solid mediumUSP36 1117 优良微生物检测规范中英文版USP36 1117 优良微生物检测规范（中英文1/2） 2013-08-09 15:30:46| 分类：USP|举报|字号订阅1117 MICROBIOLOGICAL BEST LABORATORY PRACTICES优良微生物检测规范INTRODUCTION介绍Good laboratory practices in a microbiology laboratory consis抒毒额择茎苔薄仲寇醚橱僧洲世秸诚州坦涂勾叙逾奎簇诀眯座苗尝狙吨撵值雌尖姚亢谭兵吸脓秩藏柠耳跺咒