医学专题：大鼠(Rat)Agouti相关蛋白(AGRP)-NEWA

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1、本试剂盒只能用于科学研究，不得用于医学诊断大鼠（Rat）Agouti相关蛋白（AGRP）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被Agouti相关蛋白（AGRP）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Agouti相关蛋白（AGRP）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。样品收集、处理及保存方法1. 血清：使用不含热原和内毒素的试管，操作过程中避

2、免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。自备物品1. 酶标仪（450nm）2. 高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37恒温箱操作注意事项1. 试剂盒保存在2-8，使用前室温

3、平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。2. 实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。3. 浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。5. 所有液体组分使用前充分摇匀。试剂盒组成名称96孔配置48孔配置备注微孔酶标板12孔8条12孔4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3m

4、L无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无注：标准品（S0-S5）浓度依次为：0、1.5、3、6、12、24 ng/mL试剂的准备 20洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20洗涤缓冲液加19份的蒸馏水。洗板方法1. 手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。2. 自动洗板机：每孔注入洗液350L，浸泡1min，洗板5次。操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4。2. 设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50L；3.

5、样本孔先加待测样本10L，再加样本稀释液40L；空白孔不加。4. 除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100L，用封板膜封住反应孔，37水浴锅或恒温箱温育60min。5. 弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。6. 每孔加入底物A、B各50L，37避光孵育15min。7. 每孔加入终止液50L，15min内，在450nm波长处测定各孔的OD值。结果判断 绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本

6、浓度值。试剂盒性能1. 准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。2. 灵敏度：最低检测浓度小于0.1 ng/mL。3. 特异性：不与其它可溶性结构类似物交叉反应。4. 重复性：板内、板间变异系数均小于15%。5. 贮藏：2-8，避光防潮保存。6. 有效期：6个月免责声明1. 试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。2. 严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.Rat A

7、gouti Related Protein (AGRP) ELISA Kit instructionIntended useThis AGRP ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using

8、 a spectrophotometer. In order to measure the concentration of AGRP in the sample, this AGRP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AGRP conc

9、entration. The concentration of AGRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000g. Rem

10、ove serum and assay immediately or aliquot and store samples at -20 or -80.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000g at 2-8 within 30 minutes of collection. Store samples at -20or -80. Avoid repeated

11、 freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20or -80. Avoid repeated freeze-thaw cycles.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allow

12、ed.Materials required but not supplied1. Standard microplate reader(450nm)2. Precision pipettes and Disposable pipette tips.3. 37 incubatorPrecautions1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents

13、supplied by manufacturer.2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8C in their pouch with the desiccant provided.3. Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25C)Mater

14、ials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plat

15、e membrane22User manual11Sealed bags11Note: Standard (S0 S5) concentration was followed by:0,1.5,3,6,12,24 ng/mlReagent preparation20wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1. Prepare all reagents before starting assay procedure. It is recommended that all Standard

16、s and Samples be added in duplicate to the Microelisa Stripplate.2. Add standard: Set Standard wells, testing sample wells. Add standard 50l to standard well.3. Add Sample: Add Sample 10l then add Sample Diluent 40l to testing sample well; Blank well doesnt add anyting.4. Add 100l of HRP-conjugate r

17、eagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37C. 5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400l) using a squirt bottle, manifold dispenser or autowasher. Complete rem

18、oval of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6. Add chromogen solution A 50l and chromogen solution B 50l to each well. Gently mix and incubate

19、 for 15 minutes at 37C. Protect from light.7. Add 50l Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8. Read the Optical Density (O.D.

20、) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical

21、(Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or sta

22、tistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetti

23、ng and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 0.1 ng/ml6. Standard curveStorage： 2-8.validity： six months.FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!