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1、1、剪碎叶片，放入2ml离心管中，加入2颗钢珠，加入750ul CTAB提取液，放入粉碎机进 行粉碎2、放入65摄氏度烘箱30-60mins,最好45mins3、加氯仿(500ul)4、摇匀、离心(12000r 10mins)5、离心后，吸取上清液(350-500ul)于1.5ml离心管中6、加酒精(500ul)7、放置于-20摄氏度冰箱至少30mins8、离心(12000r 6mins)9、倒掉上部液体10、放入65摄氏度烘箱2h或37摄氏度过夜11、加dd水(300-500ul)猛摇混匀，放室温2小时1, shear the blade, in 2 ml centrifuge tube,
2、add 2 steel balls, to join 750 ulCTAB extraction liquid, put it into the crusher for crushing2, placed into a 65 degrees oven for 30 to 60 mins, best 45 mins3, chloroform (500 ul)4, shake well, centrifugal (12000 r 10 mins)5After the centrifugal, draw on the fluid (350-500 ul)In 1.5 ml centrifuge tu
3、be6, add alcohol (500 ul)7, placed in refrigerator for at least 30 mins (- 20 degrees Celsius)8, centrifugal (12000 r 6 mins)9, pour out the upper liquid10, placedin a 65 degrees oven 2 h or 37 degrees Celsius for the night11, with the dd water (300-500 ul) succussion blender, put 2 hours at room temperature
水稻DNA提取方法
