荧光定量PCR引物设计软件说明书PrimerExpressguide

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17、sPrimer Express Operati on GuidePrimers 右Primers行 Forward / Reverse Primers皿誌鑑msPrimer Express Operati on Guidec/wcrrctscA GCCTCidocr rcccGAOAAG cccctttcgg ctgcttSogaCGGAGACCGA AGGGCTCTTC GG真屠炯CC CACGAACCCTAACOGMjCCT CCCGGAAGGG (jTGGCCAATG GCATCG帕辺 CTACAGTAOCTTCCCTCOGA OCOCCTTCCC CACCGGTTAC CGTAGCTC

18、CA GATOTCATGaAAAATCAACT CCAAOGTGAC CTCCCGTl|TT GCTCACWTG TTOTCACCAT TYTTAtJUG* GGTTCCACTO (jOGGCAAAA OGWTGn/C ftACACTCGTAG帕帕CCffK AACCGTGCAG ACAC0GCCAA GG畑ffTTTCC TTTGATCTGG CTCICGGCAG TTOGCACGTC rCTGCCGCTT CCTCCAAAGG AAACTACACCOACGOCCTTC ATCACCAACT TCACCTTOV! CATCCACOGT kg规JGfJTT CTGCCGGAAO T価卸TM Z

19、vGT妙ACTG GTAGCTCCCAPrimers 右Primers行 Forward / Reverse Primers皿誌鑑msPrimer Express Operati on GuidePrimers 右Primers行 Forward / Reverse Primers皿誌鑑msPrimer Express Operati on GuideTools t Find Primers/ProbesProbeForward / ReversePrimers 右Primers行 Forward / Reverse PrimersxAMxm uumm wauaiw suwww mwwxuxA

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34、00250AACGGftGEHGCCrCGWCCC卸iAGGG GTGGCAlCGfcGGT CTftCAGTACC000OC7TCCC CACOOTAacrccA qaphcat朝JOQJ5Q45DMAATCMCTCCMGQTQ轉 aCCCCnTT GCTCAChATG TT0T(XCAT500nJIAUTTGAGUTTCCACTG GAGGGCAAAA OSACTGTTAC AACAJTGGTA550爾&能鑑msPrimer Express Operati on GuideGAJGAGISCAG ACACGGCCAA iSGAfiGTHCC TTTGATGTGGCTCTCACCrc KTG

35、CCWTT ESAM35 AACTACACC60065Q750850700AtKTGCCCAA 6ACGdCCrrC AICCCAACT TCACCTTGAC CATCGACGGTTCGACiWT d似。朋屈 7A0rtWnOA ACTQQA就TO 軒畑QC第9QDtrn壮灯叔 nrrwAAm QAtuwwAd 柑3.Parameters” TabPrimer TmPrimersSequence Paramaersi Primefj /ftobe$- OideiPaiameteiValue日Piirne订mAMin Piirner I m5BRrmei Trm60Mar DiffefBnce

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37、藍Forward PrimerReverse Primer(* 不NM CXI2217MLenh 2909 hp. SdBdkm 289 boV:吕 Piimar： / Prctes Dvdei2390 Dcmhle S lraiedPaflMMp !*1 *1皿誌鑑msPrimer Express Operation Guide皿誌鑑msPrimer Express Operation GuideCATCKKGaGC AAGITOCCCT TCTA7AACCT GGGCTTTGGC AAjCAATCim ISrASXCCOG nCAMKXKA iMTATTMA CCCGAAACCG TTCn

38、iAGACTATTAT初cn CCIOGMMC ftTXCCTO3 初从CCATCG cmOCCGMT店TAT罚幽 OflACCTCHO T4J丽蠟 7CTWACC CWCI3WCCC0CAmAr(3 AfiGAinCTGA TGOTGATTTG CAGrrAGG OaiCTATGAGCGTAAATiM： TCCKW5ACT MWHXMC G冗AACTTCC OWiGATACTdijAOOTaocc wcccactoc TGAcaaarcT qqasatcm初 taccccwaCCTO：ACC03 TT閃时m ACTGCCCM CCTCTACCTC ATd0CTCTACGCTAICCT GGA

39、CCTCACC CAGAACACTI ACCiMSCACTT CTACGArSCTGCGATAGGA OCTGGAGIGG GTCnGTGAA TGGrffilGAA GATGCTMXaTCTATCC TOQT00gQ 筒戳話01$ (Mt逖购i仙 TGWCOCTTA0ACTC7AGC ACCAtmKC CGCCKACCAC CTOCWCTGT AHTOTCXiAAa.aiaa.ia乍diit&BE kg氏3SSS禺 js*:*BMlof* VWEFE3E.&O-玄二=Blicsf iA _nw I穽 f. dn倉尸 空一 MO131丄,JdM*-*5 - JWIA *SMjkwHlRS*,.

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