穴位肥大细胞及其颗粒物在针刺镇痛中的作用

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1、1纪念安徽针灸经络研究纪念安徽针灸经络研究5050周年周年 穴位肥大细胞及其颗粒物在针刺镇痛中的作用针灸经络研究回顾与展望国际学术研讨会针灸经络研究回顾与展望国际学术研讨会2Outline&穴位肥大细胞在针刺镇痛效应中的作用&穴位胶原纤维在针刺镇痛效应中的作用&穴位注射组胺的镇痛效应&手针和电针穴位信号的启动机制THE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-177Animal modelAnimal model ：AA AA (acute arthriti

2、s)(acute arthritis)RatRatComplete Freunds adjuvant Acupoint selectedAcupoint selected：Zushanli Zushanli (ST36ST36)Treat effectTreat effect：Ease painEase pain，Pain Pain thresholdthreshold各组别大鼠痛阈变化率峰值比较（各组别大鼠痛阈变化率峰值比较（2020分钟时）分钟时）(n=6)(n=6)Note:*P0.05 vs.Group A;P0.01,P0.01,P0.05 vs.Group B1;P0.05 vs.

3、Group B1;P0.01,P0.01,P0.05 vs.Group D1P0.05 vs.Group D1穴区组织肥大细胞脱颗粒率统计穴区组织肥大细胞脱颗粒率统计 (n=6)(n=6)Note:*P0.01,*P0.05 vs.Control Group;P0.01 vs.Acupuncture Group.P0.01 vs.Acupuncture Group.Degranulationof cellsTHE JOURNAL OF SCIENCE&HEALING,2008THE JOURNAL OF SCIENCE&HEALING,2008，4 4（3 3），），170-177170-17

4、7lThe acupuncture effect is positive relationship to the degranulation of mast cell at acupoint.lWhat is key factor conduce the degranulation of mast cell during acupuncture manipulation?lOur focus is on the collagen at acupoint.2022-8-213To observe the function of collagen fibers in MA analgesia.7

5、Animal groups setting:normal group(N):Normal healthy animal control group(M):Animal with AA injection of collagenase at Zusanli(ST36)group(C)manual acupuncture group with rotation manipulation(R)or lift-thrust manipulation(L),rotation group with collagenase pretreatment to destroy Zusanli(ST36)colla

6、gen fibers(CR),lift-thrust group with collagenase pretreatment to destroy Zusanli(ST36)collagen fibers(CL),lift-thrust group pretreated with collagenase to destroy Dubi(ST35)collagen fibers(CDL)(R)(L)2022-8-214Analgesia effects observed in different groups注：较正常组痛阈：注：较正常组痛阈：*；较处理前痛阈：；较处理前痛阈：；较捻转组；较捻转

7、组(R)(R)痛阈变化率：痛阈变化率：；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：；较正常组或模型组较正常组或模型组:；较捻转；较捻转组或提插组组或提插组:。2022-8-215Analgesia effects observed in different groups注：较正常组痛阈：注：较正常组痛阈：*；较处理前痛阈：；较处理前痛阈：；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：；较正常组或模型组较正常组或模型组:；较捻转；较捻转组或提插组组或提插组:。2022-8-216Analgesia effects obse

8、rved in different groups注：较正常组痛阈：注：较正常组痛阈：*；较处理前痛阈：；较处理前痛阈：；较捻转组；较捻转组(R)(R)痛阈变化率：痛阈变化率：；较捻转组较捻转组(L)(L)痛阈变化率：痛阈变化率：；较正常组或模型组较正常组或模型组:；较捻转；较捻转组或提插组组或提插组:。collagen fibers of skin and muscleMast cells in skinGroupNormalGroupInjectControlcollagen fibers of skin and muscleMast cells in skinConnective Tiss

9、ue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120Acupunc.whirling Acupunc.lift and thrust collagen fibers of skin and muscleMast cells in skincollagen fibers of skin and muscleMast cells in skinConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:1

10、10-120 Mast cells degranulation before and after collagen destroyed Collagen destroyedCollagen destroyedConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-120collagen fibers and mast cells in normal acupointcollagen fibers enlacement and mast cells degranulation whi

11、le whirlingcollagen fibers tightened and mast cells degranulated while lifting and thrustingcollagen fibers were destroyed and mast cells had no degranulationConnective Tissue Research,2008Connective Tissue Research,2008，5050（2 2）:110-120:110-1202022-8-221The mast cells in acupoint may translate the

12、 mechanical signal into the biological information of neural system.The collagen and mast cells degranulation are very important to the manual acupuncture analgesia effect.Is this the same as electric acupuncture?2022-8-222手针及电针治疗前后手针及电针治疗前后AAAA大鼠痛阈和痛阈改变率以及穴大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化区肥大细胞脱颗粒率的变化注：与注：

13、与 正常组相比较：正常组相比较：*；与；与 模型组相比较：模型组相比较：；与手针组相比较：；与手针组相比较：；与电针组相比较：与电针组相比较：。各组痛阈测量比较各组痛阈测量比较各组痛阈测量比较各组痛阈测量比较针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103各组痛阈测量比较各组痛阈测量比较针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103各组足三里穴位处脱颗粒比较各组足三里穴位处脱颗粒比较P0.01 P0.01 与空白组比较（与空白组比较（vs.controlvs.control）；）；P0.01 P0.01 与模型组比较

14、（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与与手针针刺组比较（手针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位与组胺穴位注射组比较，注射组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103各组足三里穴位处脱颗粒比较各组足三里穴位处脱颗粒比较P0.01 P0.01 与空白组比较（与空白组比较（vs.cont

15、rolvs.control）；）；P0.01 P0.01 与模型组比较（与模型组比较（vs.modelvs.model）；）；P0.01 P0.01 与与手针针刺组比较（手针针刺组比较（vs.controlvs.control）；）；P0.01 P0.01 与生理盐水穴位注射组比较；与生理盐水穴位注射组比较；P0.01 P0.01 与组胺穴位与组胺穴位注射组比较，注射组比较，P0.01 P0.01 与组胺与组胺H1H1拮抗剂穴位注射组比较。拮抗剂穴位注射组比较。针刺研究，针刺研究，20102010，3535（2 2）：）：99-10399-103l组胺参与到了针刺麻醉的穴位局部机制组胺参与到了

16、针刺麻醉的穴位局部机制l通过通过H1受体进一步传递针刺信号受体进一步传递针刺信号研究阐明了手针和电针穴位针刺有效信号的启动机制研究阐明了手针和电针穴位针刺有效信号的启动机制MAEAElectric A.10382.0000010384.00000seconds-0.199992-0.0999960.0000000.099996Volts 心电图91.20613493.14669095.08724697.027802mmHg动脉血压0.00000036.75320173.506401110.259602mmHg心室内压-41.509399-21.509399-1.50939918.4906013

17、8.490601Volts正中神经放电-0.019582-0.0093089.7E-0040.011241Volts交感神经放电10580.00000seconds-0.447150-0.247150-0.0471500.1528500.352850Volts 心电图95.61133897.52356599.435792101.348018mmHg动脉血压0.00000036.59941373.198826109.798240mmHg心室内压-13.244812-7.244812-1.2448124.75518810.755188Volts正中神经放电-0.069308-0.039308-0.0093080.0206920.050692Volts交感神经放电